Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-StbC
Location 235362..236023 Replicon chromosome
Accession NZ_CP058908
Organism Pseudomonas phenolilytica strain RBPA9

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HU825_RS01235 Protein ID WP_234302750.1
Coordinates 235604..236023 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag HU825_RS01230 Protein ID WP_077683726.1
Coordinates 235362..235607 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU825_RS01210 (HU825_01210) 231178..231576 + 399 WP_043297470.1 DoxX family protein -
HU825_RS01215 (HU825_01215) 231847..233541 + 1695 WP_234302748.1 tannase/feruloyl esterase family alpha/beta hydrolase -
HU825_RS01220 (HU825_01220) 233814..235076 + 1263 WP_234302749.1 OprD family porin -
HU825_RS01225 235165..235309 + 145 Protein_230 integrase -
HU825_RS01230 (HU825_01225) 235362..235607 + 246 WP_077683726.1 plasmid stabilization protein Antitoxin
HU825_RS01235 (HU825_01230) 235604..236023 + 420 WP_234302750.1 type II toxin-antitoxin system VapC family toxin Toxin
HU825_RS01245 (HU825_01240) 236311..237180 - 870 WP_234303433.1 DMT family transporter -
HU825_RS01250 (HU825_01245) 237219..237863 - 645 WP_008567215.1 carbonate dehydratase -
HU825_RS01255 (HU825_01250) 237964..239343 - 1380 WP_043297467.1 alpha,alpha-trehalose-phosphate synthase (UDP-forming) -
HU825_RS01260 (HU825_01255) 239384..240130 - 747 WP_234302751.1 trehalose-phosphatase -
HU825_RS01265 (HU825_01260) 240257..240799 + 543 WP_234302752.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 219365..243588 24223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15051.26 Da        Isoelectric Point: 4.4093

>T167958 WP_234302750.1 NZ_CP058908:235604-236023 [Pseudomonas phenolilytica]
MILLDTNVLSELMRAKPAPQVIEWVDAQPASELAISAITVAEILYGIARMPDGKRKQGLLDIASAMFEEDFAGNILPFDA
DAAVHYAEIAAATEAKGRIVDMADAQIAAIGRRHDAIIATRNTRHFEPLGVALIDPWSE

Download         Length: 420 bp

>T167958 NZ_CP075487:c4175134-4174961 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8977.29 Da        Isoelectric Point: 11.1585

>AT167958 WP_077683726.1 NZ_CP058908:235362-235607 [Pseudomonas phenolilytica]
MASITIRNLDDDLKAKLRVVAASHGRSMEEEARIIIRQALSRQEKRGGLGTRIRSRFATLGGAELALPERKDKARAASFD
E

Download         Length: 246 bp

>AT167958 NZ_CP075487:c4175458-4175162 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References