Detailed information of TA system TA167951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 9884..10501
• Replicon: plasmid plas4
• Accession: NZ_CP058895
• Organism: Enterococcus faecium strain ef332

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HZU50_RS14345
• Protein ID: WP_224451579.1
• Coordinates: 9884..10036 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: HZU50_RS14350
• Protein ID: WP_002305052.1
• Coordinates: 10070..10501 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZU50_RS14300 (HZU50_14300)	5216..6511	-	1296	WP_002297218.1	ISL3-like element ISEfa11 family transposase	-
HZU50_RS14305 (HZU50_14305)	6648..6792	-	145	Protein_7	type I toxin-antitoxin system toxin PepG1	-
HZU50_RS14310 (HZU50_14310)	6960..7184	+	225	WP_074399798.1	hypothetical protein	-
HZU50_RS14315 (HZU50_14315)	7632..7838	+	207	WP_002324499.1	hypothetical protein	-
HZU50_RS14320 (HZU50_14320)	7838..8089	+	252	WP_002287517.1	hypothetical protein	-
HZU50_RS14325 (HZU50_14325)	8105..8542	+	438	WP_002305049.1	hypothetical protein	-
HZU50_RS14330 (HZU50_14330)	8535..9230	+	696	WP_002305050.1	hypothetical protein	-
HZU50_RS14335 (HZU50_14335)	9395..9595	+	201	WP_002324500.1	hypothetical protein	-
HZU50_RS14340 (HZU50_14340)	9611..9790	+	180	Protein_14	hypothetical protein	-
HZU50_RS14345 (HZU50_14345)	9884..10036	+	153	WP_224451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
HZU50_RS14350 (HZU50_14350)	10070..10501	+	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HZU50_RS14355 (HZU50_14355)	10675..11628	-	954	WP_002325884.1	IS30 family transposase	-
HZU50_RS14360 (HZU50_14360)	11931..13205	+	1275	WP_002323715.1	MFS transporter	-
HZU50_RS14365 (HZU50_14365)	13259..14686	+	1428	WP_002361237.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(6')-aph(2'')	-	1..203651	203651
	-	inside	IScluster/Tn	-	-	10..11628	11618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5815.65 Da
• Isoelectric Point: 10.3560

>T167951 WP_224451579.1 NZ_CP058895:9884-10036 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 153 bp

>T167951 NZ_CP075487:c2391835-2391659 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT167951 WP_002305052.1 NZ_CP058895:10070-10501 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT167951 NZ_CP075487:c2391613-2391197 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8