Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 46235..47372 Replicon plasmid plas2
Accession NZ_CP058893
Organism Enterococcus faecium strain ef332

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag HZU50_RS13850 Protein ID WP_001284311.1
Coordinates 46509..47372 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag HZU50_RS13845 Protein ID WP_000301765.1
Coordinates 46235..46507 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZU50_RS13785 (HZU50_13785) 41824..42198 - 375 WP_002347532.1 hypothetical protein -
HZU50_RS13790 (HZU50_13790) 42220..42570 - 351 WP_002347533.1 hypothetical protein -
HZU50_RS13795 (HZU50_13795) 42587..42982 - 396 WP_002320784.1 type II toxin-antitoxin system HicB family antitoxin -
HZU50_RS13800 (HZU50_13800) 43049..43234 - 186 WP_002320785.1 type II toxin-antitoxin system HicA family toxin -
HZU50_RS13805 (HZU50_13805) 43316..43570 - 255 WP_002347534.1 hypothetical protein -
HZU50_RS13810 (HZU50_13810) 43592..43828 - 237 WP_002347535.1 hypothetical protein -
HZU50_RS13815 (HZU50_13815) 43844..44128 - 285 WP_002347536.1 hypothetical protein -
HZU50_RS13820 (HZU50_13820) 44231..44413 - 183 WP_002347537.1 hypothetical protein -
HZU50_RS13825 (HZU50_13825) 44646..44867 - 222 Protein_56 DDE-type integrase/transposase/recombinase -
HZU50_RS13830 (HZU50_13830) 44980..45660 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
HZU50_RS13835 (HZU50_13835) 45699..45911 + 213 Protein_58 chromosome partitioning protein ParA -
HZU50_RS13840 (HZU50_13840) 46003..46218 + 216 WP_001835296.1 peptide-binding protein -
HZU50_RS13845 (HZU50_13845) 46235..46507 + 273 WP_000301765.1 antitoxin Antitoxin
HZU50_RS13850 (HZU50_13850) 46509..47372 + 864 WP_001284311.1 zeta toxin family protein Toxin
HZU50_RS13855 (HZU50_13855) 47635..48372 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
HZU50_RS13860 (HZU50_13860) 48497..48580 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
HZU50_RS15410 48629..48712 - 84 Protein_64 MLS leader peptide -
HZU50_RS13865 (HZU50_13865) 49122..49916 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
HZU50_RS13870 (HZU50_13870) 50009..50260 - 252 Protein_66 GNAT family N-acetyltransferase -
HZU50_RS13875 (HZU50_13875) 50505..51800 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
HZU50_RS13880 (HZU50_13880) 51901..52035 + 135 Protein_68 IS982 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHMX / erm(B) / aph(3')-III / VanHAX - 1..87675 87675
- inside IScluster/Tn erm(B) / aph(3')-III / VanHAX - 36067..74450 38383


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T167950 WP_001284311.1 NZ_CP058893:46509-47372 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T167950 NZ_CP075487:1967859-1967962 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT167950 WP_000301765.1 NZ_CP058893:46235-46507 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT167950 NZ_CP075487:c1968000-1967729 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References