Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 508427..508998 Replicon chromosome
Accession NZ_CP058891
Organism Enterococcus faecium strain ef332

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag HZU50_RS02390 Protein ID WP_002286801.1
Coordinates 508657..508998 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag HZU50_RS02385 Protein ID WP_002323011.1
Coordinates 508427..508657 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZU50_RS02360 (HZU50_02360) 503560..504891 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
HZU50_RS02365 (HZU50_02365) 504913..505539 + 627 WP_101699687.1 cysteine hydrolase -
HZU50_RS02370 (HZU50_02370) 505722..506303 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
HZU50_RS02375 (HZU50_02375) 507035..507597 + 563 Protein_471 SOS response-associated peptidase family protein -
HZU50_RS02380 (HZU50_02380) 507802..508140 - 339 WP_002286804.1 hypothetical protein -
HZU50_RS02385 (HZU50_02385) 508427..508657 + 231 WP_002323011.1 hypothetical protein Antitoxin
HZU50_RS02390 (HZU50_02390) 508657..508998 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HZU50_RS02395 (HZU50_02395) 509848..510033 + 186 WP_002304207.1 hypothetical protein -
HZU50_RS02400 (HZU50_02400) 510538..510732 + 195 WP_002297028.1 hypothetical protein -
HZU50_RS02405 (HZU50_02405) 510923..511172 + 250 Protein_477 transposase -
HZU50_RS02410 (HZU50_02410) 511416..512246 - 831 WP_002286789.1 manganese catalase family protein -
HZU50_RS02415 (HZU50_02415) 512454..513788 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T167949 WP_002286801.1 NZ_CP058891:508657-508998 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T167949 NZ_CP075487:1803070-1803324 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT167949 WP_002323011.1 NZ_CP058891:508427-508657 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT167949 NZ_CP075487:1802822-1803073 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References