Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26881..27524 Replicon plasmid unnamed1
Accession NZ_CP058887
Organism Shigella flexneri strain STLEFF_33

Toxin (Protein)


Gene name vagD Uniprot ID A0A7W3HBH1
Locus tag HZT25_RS22660 Protein ID WP_044864680.1
Coordinates 27108..27524 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HZT25_RS22655 Protein ID WP_001261276.1
Coordinates 26881..27111 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT25_RS22630 (HZT25_22505) 22035..22811 - 777 WP_000015958.1 site-specific integrase -
HZT25_RS22635 (HZT25_22510) 22808..23551 - 744 WP_000129823.1 hypothetical protein -
HZT25_RS22640 (HZT25_22515) 23602..23952 - 351 WP_000493378.1 hypothetical protein -
HZT25_RS22645 (HZT25_22520) 24346..25419 + 1074 WP_077890912.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
HZT25_RS22650 (HZT25_22525) 25712..26626 + 915 WP_052686739.1 Abi family protein -
HZT25_RS22655 (HZT25_22530) 26881..27111 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT25_RS22660 (HZT25_22535) 27108..27524 + 417 WP_044864680.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT25_RS22665 (HZT25_22540) 27733..28713 - 981 WP_000019402.1 IS5-like element IS5 family transposase -
HZT25_RS22670 (HZT25_22545) 28914..31979 - 3066 WP_000002334.1 tetrathionate reductase subunit TtrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / qnrS1 / tet(A) - 1..95227 95227
- flank IS/Tn - - 27733..28713 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.64 Da        Isoelectric Point: 8.5403

>T167947 WP_044864680.1 NZ_CP058887:27108-27524 [Shigella flexneri]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T167947 NZ_CP075487:1058789-1059124 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT167947 WP_001261276.1 NZ_CP058887:26881-27111 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT167947 NZ_CP075487:1058541-1058789 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3HBH1

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References