Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3416595..3417213 Replicon chromosome
Accession NZ_CP058886
Organism Shigella flexneri strain STLEFF_33

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZT25_RS16675 Protein ID WP_001291435.1
Coordinates 3416995..3417213 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZT25_RS16670 Protein ID WP_000344800.1
Coordinates 3416595..3416969 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT25_RS16660 (3411684) 3411684..3412877 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HZT25_RS16665 (3412900) 3412900..3416049 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HZT25_RS16670 (3416595) 3416595..3416969 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZT25_RS16675 (3416995) 3416995..3417213 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HZT25_RS16680 (3417385) 3417385..3417936 + 552 WP_000102564.1 maltose O-acetyltransferase -
HZT25_RS16685 (3418052) 3418052..3418522 + 471 WP_000136192.1 YlaC family protein -
HZT25_RS16690 (3418686) 3418686..3420236 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT25_RS16695 (3420278) 3420278..3420631 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HZT25_RS16705 (3421010) 3421010..3421321 + 312 WP_000409911.1 MGMT family protein -
HZT25_RS16710 (3421352) 3421352..3421924 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167942 WP_001291435.1 NZ_CP058886:3416995-3417213 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167942 NZ_CP075487:184535-184642 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167942 WP_000344800.1 NZ_CP058886:3416595-3416969 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167942 NZ_CP075487:c184486-184423 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References