Detailed information of TA system TA167941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2380524..2381162
• Replicon: chromosome
• Accession: NZ_CP058886
• Organism: Shigella flexneri strain STLEFF_33

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HZT25_RS11625
• Protein ID: WP_001447010.1
• Coordinates: 2380986..2381162 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HZT25_RS11620
• Protein ID: WP_001270286.1
• Coordinates: 2380524..2380940 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT25_RS11600 (2375676)	2375676..2376617	-	942	WP_001251304.1	ABC transporter permease	-
HZT25_RS11605 (2376618)	2376618..2377631	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HZT25_RS11610 (2377649)	2377649..2378794	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HZT25_RS11615 (2379039)	2379039..2380445	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HZT25_RS11620 (2380524)	2380524..2380940	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HZT25_RS11625 (2380986)	2380986..2381162	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HZT25_RS11630 (2381384)	2381384..2381614	+	231	WP_000494244.1	YncJ family protein	-
HZT25_RS11635 (2381706)	2381706..2383667	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HZT25_RS11640 (2383740)	2383740..2384276	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HZT25_RS11645 (2384368)	2384368..2385543	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2385583..2386731	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T167941 WP_001447010.1 NZ_CP058886:c2381162-2380986 [Shigella flexneri]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T167941 NZ_CP075486:c59525-59244 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT167941 WP_001270286.1 NZ_CP058886:c2380940-2380524 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT167941 NZ_CP075486:c59766-59515 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8