Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 97102..97627 Replicon plasmid unnamed1
Accession NZ_CP058880
Organism Escherichia coli strain STLEFF_29

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HZT24_RS24055 Protein ID WP_001159871.1
Coordinates 97102..97407 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag HZT24_RS24060 Protein ID WP_000813630.1
Coordinates 97409..97627 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT24_RS24040 (93064) 93064..94230 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HZT24_RS24045 (94818) 94818..95573 - 756 WP_000852146.1 replication initiation protein RepE -
HZT24_RS24050 (96295) 96295..97101 - 807 WP_000016970.1 site-specific integrase -
HZT24_RS24055 (97102) 97102..97407 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HZT24_RS24060 (97409) 97409..97627 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HZT24_RS24065 (98187) 98187..98417 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
HZT24_RS24070 (98414) 98414..98830 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
HZT24_RS24075 (98905) 98905..100470 + 1566 WP_001128474.1 AAA family ATPase -
HZT24_RS24080 (100455) 100455..101477 + 1023 WP_000361402.1 helicase UvrD -
HZT24_RS24085 (101731) 101731..102417 - 687 Protein_118 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / lnu(F) / blaTEM-1B / sul3 / aph(3')-Ia / aac(3)-IId / sitABCD iutA / iucD / iucC / iucB / iucA 1..121453 121453


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T167918 WP_001159871.1 NZ_CP058880:c97407-97102 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T167918 NZ_CP075484:268406-268681 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT167918 WP_000813630.1 NZ_CP058880:c97627-97409 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT167918 NZ_CP075484:268678-269037 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References