Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4681351..4681967 Replicon chromosome
Accession NZ_CP058879
Organism Escherichia coli strain STLEFF_29

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag HZT24_RS22450 Protein ID WP_001129490.1
Coordinates 4681351..4681725 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag HZT24_RS22455 Protein ID WP_001275523.1
Coordinates 4681725..4681967 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT24_RS22435 (4678854) 4678854..4679756 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HZT24_RS22440 (4679753) 4679753..4680388 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HZT24_RS22445 (4680385) 4680385..4681314 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
HZT24_RS22450 (4681351) 4681351..4681725 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT24_RS22455 (4681725) 4681725..4681967 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
HZT24_RS22460 (4682187) 4682187..4682405 - 219 WP_001251290.1 CopG family transcriptional regulator -
HZT24_RS22465 (4683079) 4683079..4683993 - 915 WP_072649161.1 transposase -
HZT24_RS22470 (4684006) 4684006..4684893 - 888 Protein_4407 hypothetical protein -
HZT24_RS22475 (4685309) 4685309..4686250 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HZT24_RS22480 (4686295) 4686295..4686732 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T167913 WP_001129490.1 NZ_CP058879:c4681725-4681351 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T167913 NZ_CP075480:c3603279-3602881 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT167913 WP_001275523.1 NZ_CP058879:c4681967-4681725 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT167913 NZ_CP075480:c3603575-3603282 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References