Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4157615..4158191 Replicon chromosome
Accession NZ_CP058879
Organism Escherichia coli strain STLEFF_29

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag HZT24_RS20025 Protein ID WP_001131965.1
Coordinates 4157904..4158191 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag HZT24_RS20020 Protein ID WP_000063158.1
Coordinates 4157615..4157917 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT24_RS20000 (4152723) 4152723..4153943 + 1221 WP_000873756.1 amino acid deaminase -
HZT24_RS20005 (4154211) 4154211..4156361 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HZT24_RS20010 (4156411) 4156411..4156614 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZT24_RS20015 (4156625) 4156625..4157581 + 957 WP_001361361.1 GTPase -
HZT24_RS20020 (4157615) 4157615..4157917 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
HZT24_RS20025 (4157904) 4157904..4158191 - 288 WP_001131965.1 BrnT family toxin Toxin
HZT24_RS20030 (4158388) 4158388..4159554 + 1167 WP_000800823.1 restriction endonuclease -
HZT24_RS20035 (4159599) 4159599..4159772 + 174 Protein_3936 type I restriction-modification system subunit M N-terminal domain-containing protein -
HZT24_RS20040 (4159766) 4159766..4160434 + 669 Protein_3937 DUF3387 domain-containing protein -
HZT24_RS20045 (4160649) 4160649..4160987 + 339 WP_000132633.1 endoribonuclease SymE -
HZT24_RS20050 (4161075) 4161075..4161122 - 48 WP_229017048.1 hypothetical protein -
HZT24_RS20055 (4161370) 4161370..4162782 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T167909 WP_001131965.1 NZ_CP058879:c4158191-4157904 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167909 NZ_CP075480:2596543-2596650 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT167909 WP_000063158.1 NZ_CP058879:c4157917-4157615 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167909 NZ_CP075480:c2596496-2596430 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References