Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2014127..2014911 Replicon chromosome
Accession NZ_CP058879
Organism Escherichia coli strain STLEFF_29

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag HZT24_RS09560 Protein ID WP_000613626.1
Coordinates 2014127..2014621 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag HZT24_RS09565 Protein ID WP_001110447.1
Coordinates 2014618..2014911 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT24_RS09540 (2009320) 2009320..2010417 + 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -
HZT24_RS09545 (2010417) 2010417..2011358 + 942 WP_001305885.1 flagellar assembly peptidoglycan hydrolase FlgJ -
HZT24_RS09550 (2011424) 2011424..2013067 + 1644 WP_061092652.1 flagellar hook-associated protein FlgK -
HZT24_RS09555 (2013079) 2013079..2014032 + 954 WP_001212762.1 flagellar hook-associated protein FlgL -
HZT24_RS09560 (2014127) 2014127..2014621 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
HZT24_RS09565 (2014618) 2014618..2014911 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
HZT24_RS09570 (2015044) 2015044..2018229 - 3186 WP_061092653.1 ribonuclease E -
HZT24_RS09575 (2018802) 2018802..2019761 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T167895 WP_000613626.1 NZ_CP058879:c2014621-2014127 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T167895 NZ_CP075480:170868-170975 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT167895 WP_001110447.1 NZ_CP058879:c2014911-2014618 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT167895 NZ_CP075480:c170820-170754 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References