Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 211755..212367 Replicon chromosome
Accession NZ_CP058879
Organism Escherichia coli strain STLEFF_29

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag HZT24_RS00990 Protein ID WP_000833473.1
Coordinates 211755..211940 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1M2IVK0
Locus tag HZT24_RS00995 Protein ID WP_000499742.1
Coordinates 211957..212367 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT24_RS00975 (207221) 207221..208516 + 1296 WP_000985745.1 Fic family protein -
HZT24_RS00980 (208624) 208624..210162 + 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
HZT24_RS00985 (210203) 210203..211282 - 1080 WP_000061475.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
HZT24_RS00990 (211755) 211755..211940 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
HZT24_RS00995 (211957) 211957..212367 + 411 WP_000499742.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HZT24_RS01000 (212479) 212479..214458 - 1980 WP_001026863.1 glycoside hydrolase family 127 protein -
HZT24_RS01005 (214469) 214469..215869 - 1401 WP_000204814.1 MFS transporter -
HZT24_RS01010 (216095) 216095..216910 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T167888 WP_000833473.1 NZ_CP058879:211755-211940 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T167888 NZ_CP075477:3447968-3448186 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15242.13 Da        Isoelectric Point: 4.4482

>AT167888 WP_000499742.1 NZ_CP058879:211957-212367 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT167888 NZ_CP075477:3447568-3447942 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IVK0

References