Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 141283..141818 Replicon plasmid unnamed1
Accession NZ_CP058875
Organism Escherichia coli strain STLEFF_47

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag HZT32_RS24840 Protein ID WP_000222766.1
Coordinates 141531..141818 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag HZT32_RS24835 Protein ID WP_001132900.1
Coordinates 141283..141534 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT32_RS24790 (136410) 136410..136970 + 561 WP_039005753.1 fertility inhibition protein FinO -
HZT32_RS24795 (137106) 137106..137318 + 213 WP_001299730.1 ANR family transcriptional regulator -
HZT32_RS24800 (137564) 137564..137638 + 75 Protein_141 endonuclease -
- (137894) 137894..137955 - 62 NuclAT_1 - -
- (137894) 137894..137955 - 62 NuclAT_1 - -
- (137894) 137894..137955 - 62 NuclAT_1 - -
- (137894) 137894..137955 - 62 NuclAT_1 - -
HZT32_RS24805 (137999) 137999..138148 + 150 WP_001312851.1 Hok/Gef family protein -
HZT32_RS24810 (138432) 138432..138689 + 258 WP_000083845.1 replication regulatory protein RepA -
HZT32_RS24815 (138706) 138706..138957 - 252 WP_223195197.1 replication protein RepA -
HZT32_RS24820 (138948) 138948..138995 + 48 WP_229471593.1 hypothetical protein -
HZT32_RS24825 (138988) 138988..139470 + 483 WP_001273588.1 hypothetical protein -
HZT32_RS24830 (139463) 139463..140320 + 858 WP_000774297.1 incFII family plasmid replication initiator RepA -
HZT32_RS24835 (141283) 141283..141534 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HZT32_RS24840 (141531) 141531..141818 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / iucA / iucB / iucC / iucD / iutA / vat 1..152887 152887


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T167878 WP_000222766.1 NZ_CP058875:141531-141818 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T167878 NZ_CP075477:1365505-1365903 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT167878 WP_001132900.1 NZ_CP058875:141283-141534 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT167878 NZ_CP075477:1365278-1365505 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References