Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 58033..58676 Replicon plasmid unnamed1
Accession NZ_CP058875
Organism Escherichia coli strain STLEFF_47

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HZT32_RS24350 Protein ID WP_001034044.1
Coordinates 58260..58676 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HZT32_RS24345 Protein ID WP_001261286.1
Coordinates 58033..58263 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT32_RS24310 (53206) 53206..53373 + 168 Protein_43 colicin-B -
HZT32_RS24315 (53391) 53391..53918 - 528 WP_000203272.1 colicin B immunity protein -
HZT32_RS24320 (54162) 54162..54977 + 816 WP_001312845.1 lipid II-degrading bacteriocin colicin M -
HZT32_RS24325 (55027) 55027..55380 - 354 WP_000864812.1 colicin M immunity protein -
HZT32_RS24330 (55558) 55558..56349 - 792 WP_000016494.1 site-specific integrase -
HZT32_RS24335 (56346) 56346..57035 - 690 WP_000796229.1 hypothetical protein -
HZT32_RS24340 (57079) 57079..57429 - 351 WP_000493379.1 hypothetical protein -
HZT32_RS24345 (58033) 58033..58263 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT32_RS24350 (58260) 58260..58676 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT32_RS24355 (58751) 58751..60316 + 1566 WP_001128474.1 AAA family ATPase -
HZT32_RS24360 (60301) 60301..61323 + 1023 WP_000361402.1 helicase UvrD -
HZT32_RS24370 (62401) 62401..63315 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / iucA / iucB / iucC / iucD / iutA / vat 1..152887 152887
- flank IS/Tn sitABCD - 61348..65850 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T167870 WP_001034044.1 NZ_CP058875:58260-58676 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T167870 NZ_CP075474:c4275676-4275290 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCCGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT167870 WP_001261286.1 NZ_CP058875:58033-58263 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT167870 NZ_CP075474:c4276215-4275706 [Escherichia coli]
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGCCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGTATTG
GTGACTACTGGTACAACCTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References