Detailed information of TA system TA167869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 46892..47439
• Replicon: plasmid unnamed1
• Accession: NZ_CP058875
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HZT32_RS24275
• Protein ID: WP_097309154.1
• Coordinates: 46892..47170 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: HZT32_RS24280
• Protein ID: WP_000079941.1
• Coordinates: 47170..47439 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS26150 (43342)	43342..43476	-	135	WP_249531455.1	hypothetical protein	-
HZT32_RS24250 (43436)	43436..44377	-	942	WP_039005283.1	3-deoxy-7-phosphoheptulonate synthase	-
HZT32_RS24255 (44558)	44558..44858	+	301	Protein_32	hypothetical protein	-
HZT32_RS24260 (44936)	44936..45283	-	348	WP_000142437.1	DUF6404 family protein	-
HZT32_RS24265 (45585)	45585..45977	-	393	WP_240724491.1	hypothetical protein	-
HZT32_RS24270 (45911)	45911..46846	-	936	WP_240724492.1	3'-5' exonuclease	-
HZT32_RS24275 (46892)	46892..47170	-	279	WP_097309154.1	type II toxin-antitoxin system toxin YacB	Toxin
HZT32_RS24280 (47170)	47170..47439	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
HZT32_RS24285 (47961)	47961..49189	+	1229	WP_112030635.1	IS3-like element IS2 family transposase	-
HZT32_RS24290 (49386)	49386..50660	+	1275	WP_000489607.1	HlyD family secretion protein	-
HZT32_RS26155 (50767)	50767..51039	+	273	Protein_40	cysteine peptidase family C39 domain-containing protein	-
HZT32_RS24300 (51220)	51220..52206	+	987	Protein_41	IS4-like element IS421 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iucA / iucB / iucC / iucD / iutA / vat	1..152887	152887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10799.42 Da
• Isoelectric Point: 8.4615

>T167869 WP_097309154.1 NZ_CP058875:c47170-46892 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTVQKWP

Nucleotide

• Length: 279 bp

>T167869 NZ_CP075474:c4174065-4173892 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT167869 WP_000079941.1 NZ_CP058875:c47439-47170 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT167869 NZ_CP075474:c4174389-4174093 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5