Detailed information of TA system TA167862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2918347..2919131
• Replicon: chromosome
• Accession: NZ_CP058874
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HZT32_RS14165
• Protein ID: WP_000613624.1
• Coordinates: 2918637..2919131 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: HZT32_RS14160
• Protein ID: WP_001110446.1
• Coordinates: 2918347..2918640 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS14150 (2913496)	2913496..2914455	-	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
HZT32_RS14155 (2915028)	2915028..2918213	+	3186	WP_240724385.1	ribonuclease E	-
HZT32_RS14160 (2918347)	2918347..2918640	+	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
HZT32_RS14165 (2918637)	2918637..2919131	+	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
HZT32_RS14170 (2919226)	2919226..2920179	-	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
HZT32_RS14175 (2920191)	2920191..2921834	-	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
HZT32_RS14180 (2921900)	2921900..2922841	-	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HZT32_RS14185 (2922841)	2922841..2923938	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T167862 WP_000613624.1 NZ_CP058874:2918637-2919131 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T167862 NZ_CP075474:c2391835-2391659 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT167862 WP_001110446.1 NZ_CP058874:2918347-2918640 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT167862 NZ_CP075474:c2391613-2391197 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829G775