Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2560725..2561361 Replicon chromosome
Accession NZ_CP058874
Organism Escherichia coli strain STLEFF_47

Toxin (Protein)


Gene name hicA Uniprot ID A0A5F1EUB3
Locus tag HZT32_RS12305 Protein ID WP_000813796.1
Coordinates 2561185..2561361 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HZT32_RS12300 Protein ID WP_076838470.1
Coordinates 2560725..2561141 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT32_RS12280 (2555877) 2555877..2556818 - 942 WP_001251307.1 ABC transporter permease -
HZT32_RS12285 (2556819) 2556819..2557832 - 1014 WP_000220433.1 ABC transporter ATP-binding protein -
HZT32_RS12290 (2557850) 2557850..2558995 - 1146 WP_000047448.1 ABC transporter substrate-binding protein -
HZT32_RS12295 (2559240) 2559240..2560646 - 1407 WP_000760605.1 PLP-dependent aminotransferase family protein -
HZT32_RS12300 (2560725) 2560725..2561141 - 417 WP_076838470.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HZT32_RS12305 (2561185) 2561185..2561361 - 177 WP_000813796.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HZT32_RS12310 (2561583) 2561583..2561813 + 231 WP_000494243.1 YncJ family protein -
HZT32_RS12315 (2561905) 2561905..2563866 - 1962 WP_001317809.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HZT32_RS12320 (2563939) 2563939..2564475 - 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
HZT32_RS12325 (2564567) 2564567..2565739 + 1173 WP_001236216.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6751.78 Da        Isoelectric Point: 11.5666

>T167861 WP_000813796.1 NZ_CP058874:c2561361-2561185 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Download         Length: 177 bp

>T167861 NZ_CP075474:1967859-1967962 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15219.59 Da        Isoelectric Point: 4.5908

>AT167861 WP_076838470.1 NZ_CP058874:c2561141-2560725 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT167861 NZ_CP075474:c1968000-1967729 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5F1EUB3

Antitoxin

Source ID Structure

References