Detailed information of TA system TA167851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1388241..1388866
• Replicon: chromosome
• Accession: NZ_CP058874
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HZT32_RS06630
• Protein ID: WP_240724443.1
• Coordinates: 1388468..1388866 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HZT32_RS06625
• Protein ID: WP_000450524.1
• Coordinates: 1388241..1388468 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS06600 (1384041)	1384041..1384511	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HZT32_RS06605 (1384511)	1384511..1385083	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HZT32_RS06610 (1385229)	1385229..1386107	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HZT32_RS06615 (1386124)	1386124..1387158	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HZT32_RS06620 (1387371)	1387371..1388084	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HZT32_RS06625 (1388241)	1388241..1388468	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HZT32_RS06630 (1388468)	1388468..1388866	+	399	WP_240724443.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZT32_RS06635 (1389013)	1389013..1389876	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
HZT32_RS06640 (1389891)	1389891..1391906	+	2016	WP_000829310.1	tRNA cytosine(34) acetyltransferase TmcA	-
HZT32_RS06645 (1391980)	1391980..1392677	+	698	Protein_1304	esterase	-
HZT32_RS06650 (1392758)	1392758..1392958	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14958.25 Da
• Isoelectric Point: 8.5325

>T167851 WP_240724443.1 NZ_CP058874:1388468-1388866 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAEFARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T167851 NZ_CP075472:c4148493-4148320 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT167851 WP_000450524.1 NZ_CP058874:1388241-1388468 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT167851 NZ_CP075472:c4148817-4148521 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33