Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 953114..953768 Replicon chromosome
Accession NZ_CP058874
Organism Escherichia coli strain STLEFF_47

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HZT32_RS04675 Protein ID WP_000244781.1
Coordinates 953361..953768 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT32_RS04670 Protein ID WP_000354046.1
Coordinates 953114..953380 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT32_RS04650 (949192) 949192..950625 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
HZT32_RS04655 (950670) 950670..950981 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
HZT32_RS04660 (951145) 951145..951804 + 660 WP_000250269.1 hemolysin III family protein -
HZT32_RS04665 (951881) 951881..952861 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
HZT32_RS04670 (953114) 953114..953380 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT32_RS04675 (953361) 953361..953768 + 408 WP_000244781.1 protein YgfX Toxin
HZT32_RS04680 (953808) 953808..954329 - 522 WP_001055874.1 flavodoxin FldB -
HZT32_RS04685 (954441) 954441..955337 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZT32_RS04690 (955362) 955362..956072 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT32_RS04695 (956078) 956078..957811 + 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T167850 WP_000244781.1 NZ_CP058874:953361-953768 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167850 NZ_CP075472:c4045479-4045129 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167850 WP_000354046.1 NZ_CP058874:953114-953380 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167850 NZ_CP075472:c4045724-4045473 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References