Detailed information of TA system TA167848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 381738..382333
• Replicon: chromosome
• Accession: NZ_CP058874
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: HZT32_RS01810
• Protein ID: WP_000155159.1
• Coordinates: 381956..382333 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: HZT32_RS01805
• Protein ID: WP_000557315.1
• Coordinates: 381738..381959 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS01780 (376814)	376814..377923	+	1110	WP_001318085.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HZT32_RS01785 (377971)	377971..378897	+	927	WP_000003010.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HZT32_RS01790 (378894)	378894..380171	+	1278	WP_000803825.1	branched chain amino acid ABC transporter permease LivM	-
HZT32_RS01795 (380168)	380168..380935	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HZT32_RS01800 (380937)	380937..381650	+	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HZT32_RS01805 (381738)	381738..381959	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HZT32_RS01810 (381956)	381956..382333	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HZT32_RS01815 (382542)	382542..383858	+	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HZT32_RS01820 (384034)	384034..384921	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HZT32_RS01825 (384918)	384918..385763	+	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HZT32_RS01830 (385765)	385765..386835	+	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	371626..387575	15949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T167848 WP_000155159.1 NZ_CP058874:381956-382333 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T167848 NZ_CP075472:3644557-3644835 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT167848 WP_000557315.1 NZ_CP058874:381738-381959 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT167848 NZ_CP075472:3644294-3644554 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32