Detailed information of TA system TA167847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 354462..355093
• Replicon: chromosome
• Accession: NZ_CP058874
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: HZT32_RS01655
• Protein ID: WP_001260301.1
• Coordinates: 354462..354737 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: HZT32_RS01660
• Protein ID: WP_000593557.1
• Coordinates: 354734..355093 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS01640 (349466)	349466..350533	+	1068	WP_000361470.1	HlyD family secretion protein	-
HZT32_RS01645 (350530)	350530..353265	+	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
HZT32_RS01650 (353265)	353265..354389	+	1125	WP_001318088.1	ABC transporter permease	-
HZT32_RS01655 (354462)	354462..354737	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
HZT32_RS01660 (354734)	354734..355093	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HZT32_RS01665 (355213)	355213..355614	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
HZT32_RS01670 (355620)	355620..356426	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
HZT32_RS01675 (356423)	356423..357187	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
HZT32_RS01680 (357187)	357187..358020	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
HZT32_RS01685 (358017)	358017..358961	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T167847 WP_001260301.1 NZ_CP058874:354462-354737 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T167847 NZ_CP075472:c3638503-3638105 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT167847 WP_000593557.1 NZ_CP058874:354734-355093 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT167847 NZ_CP075472:c3638799-3638506 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1