Detailed information of TA system TA167839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5848..6373
• Replicon: plasmid unnamed3
• Accession: NZ_CP058872
• Organism: Klebsiella pneumoniae strain STLEFF_44

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: HZT31_RS27740
• Protein ID: WP_004197633.1
• Coordinates: 5848..6153 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HZT31_RS27745
• Protein ID: WP_004197642.1
• Coordinates: 6155..6373 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT31_RS27710 (HZT31_27645)	1551..2177	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
HZT31_RS27715	2174..2476	+	303	WP_004197636.1	hypothetical protein	-
HZT31_RS27720 (HZT31_27655)	2924..3718	-	795	WP_004197635.1	site-specific integrase	-
HZT31_RS27725 (HZT31_27660)	3916..4932	-	1017	WP_017899884.1	hypothetical protein	-
HZT31_RS27730 (HZT31_27665)	4943..5257	-	315	WP_053389906.1	hypothetical protein	-
HZT31_RS27735 (HZT31_27670)	5284..5679	-	396	WP_164530166.1	hypothetical protein	-
HZT31_RS27740 (HZT31_27675)	5848..6153	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
HZT31_RS27745 (HZT31_27680)	6155..6373	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HZT31_RS27750 (HZT31_27685)	6582..6788	-	207	Protein_9	hypothetical protein	-
HZT31_RS27755 (HZT31_27690)	6928..7617	+	690	WP_004193995.1	hypothetical protein	-
HZT31_RS27760 (HZT31_27695)	7649..8338	-	690	WP_020315560.1	RES family NAD+ phosphorylase	-
HZT31_RS27765 (HZT31_27700)	8775..9023	+	249	WP_000272716.1	hypothetical protein	-
HZT31_RS27770 (HZT31_27705)	9020..9592	+	573	WP_020805590.1	hypothetical protein	-
HZT31_RS27775 (HZT31_27710)	9623..10117	+	495	WP_020805591.1	hypothetical protein	-
HZT31_RS27780 (HZT31_27715)	10161..10529	+	369	WP_004197807.1	hypothetical protein	-
HZT31_RS27785 (HZT31_27720)	10563..10766	+	204	WP_004197808.1	HHA domain-containing protein	-
HZT31_RS27790 (HZT31_27725)	10780..10983	+	204	WP_004197809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103039	103039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T167839 WP_004197633.1 NZ_CP058872:c6153-5848 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T167839 NZ_CP075472:1779052-1779306 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT167839 WP_004197642.1 NZ_CP058872:c6373-6155 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT167839 NZ_CP075472:1778804-1779055 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGTGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5