Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 55083..55726 Replicon plasmid unnamed2
Accession NZ_CP058871
Organism Klebsiella pneumoniae strain STLEFF_44

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HZT31_RS27365 Protein ID WP_000754566.1
Coordinates 55083..55499 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HZT31_RS27370 Protein ID WP_001261276.1
Coordinates 55496..55726 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT31_RS27345 (HZT31_27280) 50446..51366 + 921 Protein_58 BREX system Lon protease-like protein BrxL -
HZT31_RS27350 (HZT31_27285) 51474..51932 - 459 Protein_59 IS3-like element ISEc15 family transposase -
HZT31_RS27355 (HZT31_27290) 52013..53521 - 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
HZT31_RS27360 (HZT31_27295) 54194..54879 - 686 Protein_61 IS3-like element ISEc15 family transposase -
HZT31_RS27365 (HZT31_27300) 55083..55499 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT31_RS27370 (HZT31_27305) 55496..55726 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT31_RS27375 (HZT31_27310) 56300..56650 + 351 WP_000493378.1 hypothetical protein -
HZT31_RS27380 (HZT31_27315) 56701..57444 + 744 WP_000129823.1 hypothetical protein -
HZT31_RS27385 (HZT31_27320) 57441..58217 + 777 WP_000015958.1 site-specific integrase -
HZT31_RS27390 (HZT31_27325) 58275..58532 - 258 WP_000764642.1 hypothetical protein -
HZT31_RS27395 (HZT31_27330) 58661..58765 - 105 WP_032409716.1 hypothetical protein -
HZT31_RS27400 (HZT31_27335) 59300..60166 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / blaCTX-M-15 / blaTEM-1B / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(3'')-Ib / aph(6)-Id / floR / catA2 / tet(D) / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / qnrB52 - 1..115053 115053


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T167838 WP_000754566.1 NZ_CP058871:c55499-55083 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T167838 NZ_CP075472:1778804-1779055 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGTGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT167838 WP_001261276.1 NZ_CP058871:c55726-55496 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT167838 NZ_CP075472:1779052-1779306 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References