Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5170198..5170823 Replicon chromosome
Accession NZ_CP058869
Organism Klebsiella pneumoniae strain STLEFF_44

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag HZT31_RS25230 Protein ID WP_019705794.1
Coordinates 5170198..5170581 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HZT31_RS25235 Protein ID WP_004150355.1
Coordinates 5170581..5170823 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT31_RS25215 (5167564) 5167564..5168466 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HZT31_RS25220 (5168463) 5168463..5169098 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HZT31_RS25225 (5169095) 5169095..5170024 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HZT31_RS25230 (5170198) 5170198..5170581 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT31_RS25235 (5170581) 5170581..5170823 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HZT31_RS25240 (5171028) 5171028..5171945 + 918 WP_004146235.1 alpha/beta hydrolase -
HZT31_RS25245 (5171960) 5171960..5172901 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HZT31_RS25250 (5172946) 5172946..5173383 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HZT31_RS25255 (5173380) 5173380..5174240 - 861 WP_004146232.1 virulence factor BrkB family protein -
HZT31_RS25260 (5174234) 5174234..5174833 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T167835 WP_019705794.1 NZ_CP058869:c5170581-5170198 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T167835 NZ_CP075472:847098-847505 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT167835 WP_004150355.1 NZ_CP058869:c5170823-5170581 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT167835 NZ_CP075472:846851-847117 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References