Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 57627..58270 Replicon plasmid unnamed2
Accession NZ_CP058868
Organism Klebsiella pneumoniae strain STLEFF_42

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HZT30_RS27405 Protein ID WP_000754566.1
Coordinates 57627..58043 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HZT30_RS27410 Protein ID WP_001261276.1
Coordinates 58040..58270 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT30_RS27385 (HZT30_27290) 54485..54568 - 84 Protein_61 SOS response-associated peptidase -
HZT30_RS27390 55088..55189 + 102 Protein_62 Tn3 family transposase -
HZT30_RS27395 (HZT30_27295) 55209..56285 - 1077 WP_000227969.1 IS110 family transposase -
HZT30_RS27400 (HZT30_27300) 56567..57423 - 857 Protein_64 IS3-like element ISEc15 family transposase -
HZT30_RS27405 (HZT30_27305) 57627..58043 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT30_RS27410 (HZT30_27310) 58040..58270 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT30_RS27415 (HZT30_27315) 58844..59194 + 351 WP_000493378.1 hypothetical protein -
HZT30_RS27420 (HZT30_27320) 59245..59988 + 744 WP_000129823.1 hypothetical protein -
HZT30_RS27425 (HZT30_27325) 59985..60761 + 777 WP_000015958.1 site-specific integrase -
HZT30_RS27430 (HZT30_27330) 60819..61076 - 258 WP_000764642.1 hypothetical protein -
HZT30_RS27435 (HZT30_27335) 61205..61309 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / mph(A) / sul1 / qnrB52 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / floR / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib - 1..62003 62003


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T167818 WP_000754566.1 NZ_CP058868:c58043-57627 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T167818 NZ_CP075469:1019743-1020078 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT167818 WP_001261276.1 NZ_CP058868:c58270-58040 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT167818 NZ_CP075469:1019495-1019743 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References