Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4662254..4663064 Replicon chromosome
Accession NZ_CP058866
Organism Klebsiella pneumoniae strain STLEFF_42

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HZT30_RS22795 Protein ID WP_004178461.1
Coordinates 4662254..4662787 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HZT30_RS22800 Protein ID WP_002887278.1
Coordinates 4662798..4663064 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT30_RS22790 (4661085) 4661085..4662206 + 1122 WP_002887282.1 cupin domain-containing protein -
HZT30_RS22795 (4662254) 4662254..4662787 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HZT30_RS22800 (4662798) 4662798..4663064 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HZT30_RS22805 (4663167) 4663167..4664600 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
HZT30_RS22810 (4664590) 4664590..4665273 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HZT30_RS22815 (4665445) 4665445..4666830 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
HZT30_RS22820 (4666848) 4666848..4667192 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T167813 WP_004178461.1 NZ_CP058866:c4662787-4662254 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T167813 NZ_CP075469:205480-205587 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT167813 WP_002887278.1 NZ_CP058866:c4663064-4662798 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT167813 NZ_CP075469:c205432-205366 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References