Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 67039..67691 Replicon plasmid unnamed1
Accession NZ_CP058860
Organism Klebsiella quasipneumoniae strain STLEFF_28

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag HZT23_RS25755 Protein ID WP_017901321.1
Coordinates 67266..67691 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag HZT23_RS25750 Protein ID WP_001261275.1
Coordinates 67039..67269 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT23_RS25745 (HZT23_25630) 64210..66786 + 2577 WP_017901322.1 nuclease domain-containing protein -
HZT23_RS25750 (HZT23_25635) 67039..67269 + 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT23_RS25755 (HZT23_25640) 67266..67691 + 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT23_RS25760 (HZT23_25645) 67709..68677 + 969 WP_077254762.1 IS5 family transposase -
HZT23_RS25765 (HZT23_25650) 68736..69272 + 537 Protein_76 integrase core domain-containing protein -
HZT23_RS25770 69325..69498 + 174 Protein_77 nuclease -
HZT23_RS25775 (HZT23_25655) 69685..71241 + 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
HZT23_RS25780 71333..71560 - 228 Protein_79 IS3 family transposase -
HZT23_RS25785 71571..71741 + 171 Protein_80 LysR family transcriptional regulator -
HZT23_RS25790 (HZT23_25665) 71866..71982 - 117 Protein_81 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / tet(D) - 1..204335 204335
- flank IS/Tn - - 67709..68677 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T167796 WP_017901321.1 NZ_CP058860:67266-67691 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T167796 NZ_CP075465:854451-854858 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT167796 WP_001261275.1 NZ_CP058860:67039-67269 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT167796 NZ_CP075465:854204-854470 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References