Detailed information of TA system TA167794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4619867..4620603
• Replicon: chromosome
• Accession: NZ_CP058859
• Organism: Klebsiella quasipneumoniae strain STLEFF_28

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HZT23_RS22480
• Protein ID: WP_032432065.1
• Coordinates: 4620121..4620603 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HZT23_RS22475
• Protein ID: WP_003026799.1
• Coordinates: 4619867..4620133 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT23_RS22465 (4615749)	4615749..4617272	-	1524	WP_240708890.1	EAL domain-containing protein	-
HZT23_RS22475 (4619867)	4619867..4620133	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HZT23_RS22480 (4620121)	4620121..4620603	+	483	WP_032432065.1	GNAT family N-acetyltransferase	Toxin
HZT23_RS22485 (4620762)	4620762..4622294	-	1533	WP_240708892.1	IS3-like element ISKpn38 family transposase	-
HZT23_RS22490 (4622358)	4622358..4622807	+	450	Protein_4411	integrase core domain-containing protein	-
HZT23_RS22495 (4622860)	4622860..4623033	+	174	Protein_4412	nuclease	-
HZT23_RS22500 (4623220)	4623220..4624776	+	1557	WP_224252876.1	sensor domain-containing diguanylate cyclase	-
HZT23_RS22505 (4625230)	4625230..4625343	-	114	Protein_4414	transposase	-
HZT23_RS22510 (4625435)	4625435..4625566	+	132	Protein_4415	IS5/IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4619045..4625343	6298
	-	inside	Genomic island	-	-	4601832..4648377	46545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17268.93 Da
• Isoelectric Point: 8.7400

>T167794 WP_032432065.1 NZ_CP058859:4620121-4620603 [Klebsiella quasipneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T167794 NZ_CP075465:698943-699320 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT167794 WP_003026799.1 NZ_CP058859:4619867-4620133 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT167794 NZ_CP075465:698485-698853 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66