Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36052..36695 Replicon plasmid unnamed1
Accession NZ_CP058858
Organism Klebsiella pneumoniae strain STLE2

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HZT19_RS26995 Protein ID WP_000754566.1
Coordinates 36052..36468 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HZT19_RS27000 Protein ID WP_001261276.1
Coordinates 36465..36695 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT19_RS26970 (HZT19_26905) 31454..32158 - 705 WP_001067834.1 IS6-like element IS26 family transposase -
HZT19_RS26975 (HZT19_26910) 32226..32747 + 522 Protein_35 Tn3 family transposase -
HZT19_RS26980 (HZT19_26915) 32910..32993 - 84 Protein_36 SOS response-associated peptidase -
HZT19_RS26985 (HZT19_26920) 33634..34710 - 1077 WP_000227969.1 IS110 family transposase -
HZT19_RS26990 (HZT19_26925) 34992..35848 - 857 Protein_38 IS3-like element ISEc15 family transposase -
HZT19_RS26995 (HZT19_26930) 36052..36468 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT19_RS27000 (HZT19_26935) 36465..36695 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT19_RS27005 (HZT19_26940) 37269..37619 + 351 WP_000493378.1 hypothetical protein -
HZT19_RS27010 (HZT19_26945) 37670..38413 + 744 WP_000129823.1 hypothetical protein -
HZT19_RS27015 (HZT19_26950) 38410..39186 + 777 WP_000015958.1 site-specific integrase -
HZT19_RS27020 (HZT19_26955) 39244..39501 - 258 WP_000764642.1 hypothetical protein -
HZT19_RS27025 (HZT19_26960) 39630..39734 - 105 WP_032409716.1 hypothetical protein -
HZT19_RS27030 (HZT19_26965) 40264..41130 + 867 WP_004118283.1 replication initiation protein -
HZT19_RS27035 (HZT19_26970) 41307..41576 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia - 1..43449 43449


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T167779 WP_000754566.1 NZ_CP058858:c36468-36052 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T167779 NZ_CP075462:c1893363-1892989 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT167779 WP_001261276.1 NZ_CP058858:c36695-36465 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT167779 NZ_CP075462:c1893820-1893452 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References