Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5379901..5380526 Replicon chromosome
Accession NZ_CP058857
Organism Klebsiella pneumoniae strain STLE2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HZT19_RS26435 Protein ID WP_002882817.1
Coordinates 5379901..5380284 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HZT19_RS26440 Protein ID WP_004150355.1
Coordinates 5380284..5380526 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT19_RS26420 (HZT19_26355) 5377267..5378169 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HZT19_RS26425 (HZT19_26360) 5378166..5378801 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HZT19_RS26430 (HZT19_26365) 5378798..5379727 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HZT19_RS26435 (HZT19_26370) 5379901..5380284 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT19_RS26440 (HZT19_26375) 5380284..5380526 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HZT19_RS26445 (HZT19_26380) 5380731..5381648 + 918 WP_002882812.1 alpha/beta hydrolase -
HZT19_RS26450 (HZT19_26385) 5381662..5382603 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HZT19_RS26455 (HZT19_26390) 5382648..5383085 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HZT19_RS26460 (HZT19_26395) 5383082..5383942 - 861 WP_002882807.1 virulence factor BrkB family protein -
HZT19_RS26465 (HZT19_26400) 5383936..5384535 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T167778 WP_002882817.1 NZ_CP058857:c5380284-5379901 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T167778 NZ_CP075462:1019743-1020078 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT167778 WP_004150355.1 NZ_CP058857:c5380526-5380284 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT167778 NZ_CP075462:1019495-1019743 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References