Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26881..27524 Replicon plasmid unnamed1
Accession NZ_CP058854
Organism Shigella flexneri strain STLEFF_38

Toxin (Protein)


Gene name vagD Uniprot ID A0A7W3HBH1
Locus tag HZT29_RS22685 Protein ID WP_044864680.1
Coordinates 27108..27524 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HZT29_RS22680 Protein ID WP_001261276.1
Coordinates 26881..27111 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT29_RS22655 (HZT29_22435) 22035..22811 - 777 WP_000015958.1 site-specific integrase -
HZT29_RS22660 (HZT29_22440) 22808..23551 - 744 WP_000129823.1 hypothetical protein -
HZT29_RS22665 (HZT29_22445) 23602..23952 - 351 WP_000493378.1 hypothetical protein -
HZT29_RS22670 (HZT29_22450) 24346..25419 + 1074 WP_077890912.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
HZT29_RS22675 (HZT29_22455) 25712..26626 + 915 WP_052686739.1 Abi family protein -
HZT29_RS22680 (HZT29_22460) 26881..27111 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT29_RS22685 (HZT29_22465) 27108..27524 + 417 WP_044864680.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT29_RS22690 (HZT29_22470) 27733..28713 - 981 WP_000019402.1 IS5-like element IS5 family transposase -
HZT29_RS22695 (HZT29_22475) 28759..29865 - 1107 WP_240765056.1 Dyp-type peroxidase -
HZT29_RS22700 (HZT29_22480) 29917..30288 - 372 Protein_30 NAD(P)-dependent oxidoreductase -
HZT29_RS22705 (HZT29_22485) 30346..31043 + 698 WP_240765058.1 IS1 family transposase -
HZT29_RS22710 (HZT29_22490) 31177..31605 + 429 WP_001554382.1 GFA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaTEM-1B / blaLAP-2 / qnrS1 / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / floR - 1..122103 122103


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.64 Da        Isoelectric Point: 8.5403

>T167758 WP_044864680.1 NZ_CP058854:27108-27524 [Shigella flexneri]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T167758 NZ_CP075452:c2620204-2618882 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCACTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT167758 WP_001261276.1 NZ_CP058854:26881-27111 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT167758 NZ_CP075452:c2620470-2620204 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3HBH1

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References