Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3548351..3549005 Replicon chromosome
Accession NZ_CP058853
Organism Shigella flexneri strain STLEFF_38

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZT29_RS17090 Protein ID WP_000244777.1
Coordinates 3548351..3548758 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT29_RS17095 Protein ID WP_000354046.1
Coordinates 3548739..3549005 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT29_RS17070 (3544308) 3544308..3546041 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
HZT29_RS17075 (3546047) 3546047..3546757 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT29_RS17080 (3546782) 3546782..3547678 - 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
HZT29_RS17085 (3547790) 3547790..3548311 + 522 WP_001055874.1 flavodoxin FldB -
HZT29_RS17090 (3548351) 3548351..3548758 - 408 WP_000244777.1 protein YgfX Toxin
HZT29_RS17095 (3548739) 3548739..3549005 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT29_RS17100 (3549248) 3549248..3550228 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZT29_RS17105 (3550424) 3550424..3551083 - 660 WP_000250274.1 hemolysin III family protein -
HZT29_RS17110 (3551247) 3551247..3551558 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HZT29_RS17115 (3551603) 3551603..3553036 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HZT29_RS17120 (3553093) 3553093..3553836 - 744 WP_239637467.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167753 WP_000244777.1 NZ_CP058853:c3548758-3548351 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167753 NZ_CP075452:327387-327755 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167753 WP_000354046.1 NZ_CP058853:c3549005-3548739 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167753 NZ_CP075452:327169-327390 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References