Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1003364..1003982 Replicon chromosome
Accession NZ_CP058853
Organism Shigella flexneri strain STLEFF_38

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZT29_RS04780 Protein ID WP_001291435.1
Coordinates 1003364..1003582 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZT29_RS04785 Protein ID WP_000344800.1
Coordinates 1003608..1003982 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT29_RS04740 (998480) 998480..998791 - 312 WP_000409911.1 MGMT family protein -
HZT29_RS04750 (999191) 999191..999523 + 333 WP_001741293.1 DUF1428 family protein -
HZT29_RS04755 (999565) 999565..1000730 - 1166 Protein_926 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT29_RS04760 (1000786) 1000786..1001483 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
HZT29_RS04765 (1001499) 1001499..1001891 - 393 Protein_928 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT29_RS04770 (1002055) 1002055..1002525 - 471 WP_000136192.1 YlaC family protein -
HZT29_RS04775 (1002641) 1002641..1003192 - 552 WP_000102564.1 maltose O-acetyltransferase -
HZT29_RS04780 (1003364) 1003364..1003582 - 219 WP_001291435.1 HHA domain-containing protein Toxin
HZT29_RS04785 (1003608) 1003608..1003982 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZT29_RS04790 (1004528) 1004528..1007677 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HZT29_RS04795 (1007700) 1007700..1008893 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167735 WP_001291435.1 NZ_CP058853:c1003582-1003364 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167735 NZ_CP075374:1084198-1084533 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167735 WP_000344800.1 NZ_CP058853:c1003982-1003608 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167735 NZ_CP075374:1083950-1084198 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References