Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 455750..456326 Replicon chromosome
Accession NZ_CP058853
Organism Shigella flexneri strain STLEFF_38

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZT29_RS02145 Protein ID WP_001297643.1
Coordinates 455750..456037 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag HZT29_RS02150 Protein ID WP_000063160.1
Coordinates 456024..456326 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT29_RS02130 (451987) 451987..453267 - 1281 WP_001037390.1 DUF445 domain-containing protein -
HZT29_RS02135 (453308) 453308..454540 - 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
HZT29_RS02140 (454864) 454864..455551 + 688 Protein_414 IS1 family transposase -
HZT29_RS02145 (455750) 455750..456037 + 288 WP_001297643.1 BrnT family toxin Toxin
HZT29_RS02150 (456024) 456024..456326 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
HZT29_RS02155 (456360) 456360..457316 - 957 WP_001297640.1 GTPase -
HZT29_RS02160 (457327) 457327..457530 - 204 WP_000467859.1 YbdD/YjiX family protein -
HZT29_RS02165 (457580) 457580..459730 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 455342..455551 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167731 WP_001297643.1 NZ_CP058853:455750-456037 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167731 NZ_CP075374:311527-311757 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT167731 WP_000063160.1 NZ_CP058853:456024-456326 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167731 NZ_CP075374:311754-312113 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References