Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 273179..273676 Replicon chromosome
Accession NZ_CP058853
Organism Shigella flexneri strain STLEFF_38

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HZT29_RS01250 Protein ID WP_001173343.1
Coordinates 273503..273676 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HZT29_RS01245 Protein ID WP_000398619.1
Coordinates 273179..273475 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT29_RS01225 (269910) 269910..270629 - 720 WP_000611288.1 two-component system response regulator DcuR -
HZT29_RS01230 (270626) 270626..272257 - 1632 WP_001216477.1 sensor histidine kinase -
HZT29_RS01235 (272438) 272438..272668 + 231 WP_024250366.1 4Fe-4S mono-cluster protein YjdI -
HZT29_RS01240 (272680) 272680..272952 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
HZT29_RS01245 (273179) 273179..273475 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HZT29_RS01250 (273503) 273503..273676 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HZT29_RS01255 (273795) 273795..275312 - 1518 WP_001295074.1 lysine--tRNA ligase -
HZT29_RS01260 (275549) 275549..277006 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167729 WP_001173343.1 NZ_CP058853:273503-273676 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167729 NZ_CP075374:236378-236485 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT167729 WP_000398619.1 NZ_CP058853:273179-273475 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167729 NZ_CP075374:c236321-236266 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References