Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4164284..4164781 Replicon chromosome
Accession NZ_CP058849
Organism Shigella flexneri strain STLEFF_36

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HZT28_RS20340 Protein ID WP_001173343.1
Coordinates 4164284..4164457 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HZT28_RS20345 Protein ID WP_000398619.1
Coordinates 4164485..4164781 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT28_RS20330 (4160954) 4160954..4162411 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HZT28_RS20335 (4162648) 4162648..4164165 + 1518 WP_001295074.1 lysine--tRNA ligase -
HZT28_RS20340 (4164284) 4164284..4164457 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HZT28_RS20345 (4164485) 4164485..4164781 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HZT28_RS20350 (4165008) 4165008..4165280 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
HZT28_RS20355 (4165292) 4165292..4165522 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HZT28_RS20360 (4165703) 4165703..4167334 + 1632 WP_001216477.1 sensor histidine kinase -
HZT28_RS20365 (4167331) 4167331..4168050 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167728 WP_001173343.1 NZ_CP058849:c4164457-4164284 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167728 NZ_CP075374:235895-236002 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT167728 WP_000398619.1 NZ_CP058849:c4164781-4164485 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167728 NZ_CP075374:c235846-235783 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References