Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3416414..3417032 Replicon chromosome
Accession NZ_CP058849
Organism Shigella flexneri strain STLEFF_36

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZT28_RS16700 Protein ID WP_001291435.1
Coordinates 3416814..3417032 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZT28_RS16695 Protein ID WP_000344800.1
Coordinates 3416414..3416788 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT28_RS16685 (3411503) 3411503..3412696 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HZT28_RS16690 (3412719) 3412719..3415868 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HZT28_RS16695 (3416414) 3416414..3416788 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZT28_RS16700 (3416814) 3416814..3417032 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HZT28_RS16705 (3417204) 3417204..3417755 + 552 WP_000102564.1 maltose O-acetyltransferase -
HZT28_RS16710 (3417871) 3417871..3418341 + 471 WP_000136192.1 YlaC family protein -
HZT28_RS16715 (3418505) 3418505..3420055 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT28_RS16720 (3420097) 3420097..3420450 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HZT28_RS16730 (3420829) 3420829..3421140 + 312 WP_000409911.1 MGMT family protein -
HZT28_RS16735 (3421171) 3421171..3421743 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167724 WP_001291435.1 NZ_CP058849:3416814-3417032 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167724 NZ_CP075372:c4835283-4834972 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167724 WP_000344800.1 NZ_CP058849:3416414-3416788 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167724 NZ_CP075372:c4834971-4834681 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References