Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 812786..813440 Replicon chromosome
Accession NZ_CP058849
Organism Shigella flexneri strain STLEFF_36

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZT28_RS03995 Protein ID WP_000244777.1
Coordinates 813033..813440 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT28_RS03990 Protein ID WP_000354046.1
Coordinates 812786..813052 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT28_RS03965 (807955) 807955..808698 + 744 WP_000951962.1 SDR family oxidoreductase -
HZT28_RS03970 (808755) 808755..810188 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HZT28_RS03975 (810233) 810233..810544 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HZT28_RS03980 (810708) 810708..811367 + 660 WP_000250274.1 hemolysin III family protein -
HZT28_RS03985 (811563) 811563..812543 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZT28_RS03990 (812786) 812786..813052 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT28_RS03995 (813033) 813033..813440 + 408 WP_000244777.1 protein YgfX Toxin
HZT28_RS04000 (813480) 813480..814001 - 522 WP_001055874.1 flavodoxin FldB -
HZT28_RS04005 (814113) 814113..815009 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZT28_RS04010 (815034) 815034..815744 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT28_RS04015 (815750) 815750..817483 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167710 WP_000244777.1 NZ_CP058849:813033-813440 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167710 NZ_CP075371:c1732300-1731944 [Nocardioides aquaticus]
GTGGTCATCGACGAGGGCGCATCTCCGATTCCCGGCGTTCTGGCCATCAGTGCGGTGACACTCGCAGAGCTGCACTTCGG
CGTCCTGGTTGCCAAGACCACCGAGGTGCGCGCGGAGCGGCTGCGTCGGCTCAGCGTGCTCCAGCAGCGCTTCGACGCCC
TGCCGGTCGATGACGCGGTCGCCGCCAGCTACGGCCGTCTGTCGGCGGCCGTCGTCGACGCCGGGCGACGCCCCCGGGGC
CGTGTGATGGACCTCCTGATCGCGGCCACTGCCCACGCGCACGGCGCCCGGCTGTACACCCGCAACCCGGTCGACTTCGC
CGGCCTCGAGGAGCTCGTCGACGTCGTCGGCGTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167710 WP_000354046.1 NZ_CP058849:812786-813052 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167710 NZ_CP075371:c1732605-1732357 [Nocardioides aquaticus]
ATGACGACCGTCGGCTTGCGTGAGCTGAGGCAGGACGCGTCCGAACTCGTCCGTCGCGTCGAGGCAGGCGAGGAGATCAC
GATCTCCGTCTCTGGACGTCCCAGCGCTGTCTTGGTGCCCGTGCGGCCCACCCGCTGGAGGACCTGGCGGGAGGTCGAGG
ATCTGTTTCGCGGTCCCGCCGACCCAGACTGGGCGCAGGACCGAGCGCTCGTCGACGACGAGCTGTGTGACCCTTGGGCC
GGTCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References