Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3525317..3525971 Replicon chromosome
Accession NZ_CP058846
Organism Shigella flexneri strain STLEFF_35

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag HZT27_RS17340 Protein ID WP_000244783.1
Coordinates 3525317..3525724 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT27_RS17345 Protein ID WP_000354046.1
Coordinates 3525705..3525971 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT27_RS17320 (3521274) 3521274..3523007 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
HZT27_RS17325 (3523013) 3523013..3523723 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT27_RS17330 (3523748) 3523748..3524644 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZT27_RS17335 (3524756) 3524756..3525277 + 522 WP_001055874.1 flavodoxin FldB -
HZT27_RS17340 (3525317) 3525317..3525724 - 408 WP_000244783.1 protein YgfX Toxin
HZT27_RS17345 (3525705) 3525705..3525971 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT27_RS17350 (3526214) 3526214..3527194 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZT27_RS17355 (3527390) 3527390..3528049 - 660 WP_000250274.1 hemolysin III family protein -
HZT27_RS17360 (3528213) 3528213..3528524 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HZT27_RS17365 (3528569) 3528569..3530002 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HZT27_RS17370 (3530059) 3530059..3530802 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T167700 WP_000244783.1 NZ_CP058846:c3525724-3525317 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167700 NZ_CP075345:961825-962235 [Enterobacter kobei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCGTTAATGCTCCACGGCCTGGTTGCGGC
CATCGTGTTACTCATGCCATGGCCCCTGAGCTATACGCCGCTGTGGCTGCTGTTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCTCGTCAGGGTGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTGGGCATGCCATGGATGCTCAACTCCGGCATGATGTTACGGTTACGCAATGTGGACGGCGGGCGTTG
CCAGCACCTGTGGCTGGCGGCTGACAGTATGGATGCCTCAGAATGGCGAGATCTGCGCCGGATGTTGTTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167700 WP_000354046.1 NZ_CP058846:c3525971-3525705 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167700 NZ_CP075345:961578-961844 [Enterobacter kobei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGGCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCAGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References