Detailed information of TA system TA167699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3434376..3434959
• Replicon: chromosome
• Accession: NZ_CP058846
• Organism: Shigella flexneri strain STLEFF_35

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HZT27_RS16935
• Protein ID: WP_240766991.1
• Coordinates: 3434376..3434711 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: HZT27_RS16940
• Protein ID: WP_000581941.1
• Coordinates: 3434711..3434959 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT27_RS16920 (3430263)	3430263..3431561	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
HZT27_RS16925 (3431649)	3431649..3433286	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HZT27_RS16930 (3433514)	3433514..3434305	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
HZT27_RS16935 (3434376)	3434376..3434711	-	336	WP_240766991.1	endoribonuclease MazF	Toxin
HZT27_RS16940 (3434711)	3434711..3434959	-	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HZT27_RS16945 (3435037)	3435037..3437271	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
HZT27_RS16950 (3437319)	3437319..3438620	-	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12110.09 Da
• Isoelectric Point: 8.2618

>T167699 WP_240766991.1 NZ_CP058846:c3434711-3434376 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCITQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T167699 NZ_CP075345:c808330-807953 [Enterobacter kobei]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT167699 WP_000581941.1 NZ_CP058846:c3434959-3434711 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT167699 NZ_CP075345:c808739-808380 [Enterobacter kobei]
TTGTCGAATACAATACCGCCTGTAAACCACGATATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTT
TGGCGCACGTCTGGTGCAGGAGGGCAACCGGCTGCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACG
CGGATTTACGCCATCTCGACCAGGCATTCCCGCTACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGT
CTATCTCGCAATTTACCCGACACCTGCAACAACAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9