Detailed information of TA system TA167689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2081066..2082654
• Replicon: chromosome
• Accession: NZ_CP058846
• Organism: Shigella flexneri strain STLEFF_35

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: HZT27_RS09980
• Protein ID: WP_001125439.1
• Coordinates: 2081066..2082388 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HZT27_RS09985
• Protein ID: WP_001301023.1
• Coordinates: 2082388..2082654 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT27_RS09970 (2079141)	2079141..2079851	-	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-
HZT27_RS09975 (2080212)	2080212..2080694	-	483	WP_000931227.1	fimbrial protein	-
HZT27_RS09980 (2081066)	2081066..2082388	-	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HZT27_RS09985 (2082388)	2082388..2082654	-	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HZT27_RS09990 (2082877)	2082877..2084457	-	1581	Protein_1950	autotransporter outer membrane beta-barrel domain-containing protein	-
HZT27_RS09995 (2084453)	2084453..2085229	+	777	Protein_1951	histidine kinase	-
HZT27_RS10000 (2085241)	2085241..2086263	+	1023	WP_240767121.1	autoinducer 2 ABC transporter substrate-binding protein LsrB	-
HZT27_RS10005 (2086290)	2086290..2087165	+	876	WP_000774188.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-
HZT27_RS10010 (2087189)	2087189..2087479	+	291	WP_000558527.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2066884..2082654	15770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T167689 WP_001125439.1 NZ_CP058846:c2082388-2081066 [Shigella flexneri]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T167689 NZ_CP075343:c2528604-2528257 [Streptomyces sp. EMB24]
ATGAGGCGTGGTGACATCTACATGGTGGACCTGGAGCCGGTCAGGGGCAGCGAGGCCAACAAGGTCAGGCCCGCCGTGAT
CGTGTCCAACGACGGCGCCAACGCATCCGTCGAGCGGCACGGACGCGGTGTCGTCAGCGTGGTGCCGCTGACGTCGAACA
CCACGCGAGTCCTGTCGTTCCAGGTCTTCCTGAGCGCGGACGAGAGCCGGCTGCCCAAGGACTCCAAGGCGCAGTGCGAG
CAGGTGCGCGCCGTCGCCCCCGACCGTCTGCTGCACCGGGTCGGTGCTGTCCCCCGGCAGCGGATGGCCGACATCGACGT
GGCGCTGCGGCGGCACCTGGCGCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT167689 WP_001301023.1 NZ_CP058846:c2082654-2082388 [Shigella flexneri]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT167689 NZ_CP075343:c2528821-2528591 [Streptomyces sp. EMB24]
ATGAAGATCAGTGTGAGCCTGCCGCAGGAGGATGTCGCCTTCGTCGACGAATACGCCGCACGCACCGACGCCGAGTCCAG
GTCGGCCGTGATACACGCCGCGATCGAGCTGCTGCGACAGGCGCAGCTCGTACAGGAGTACACCGAGGCGTTCGCCGAGT
GGGACGCCGGTGAGGACGCCGCCGCCTGGGACCGGACGAGTGGGGACGGGGTCGCCGATGAGGCGTGGTGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU