Detailed information of TA system TA167688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1990770..1991408
• Replicon: chromosome
• Accession: NZ_CP058846
• Organism: Shigella flexneri strain STLEFF_35

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HZT27_RS09595
• Protein ID: WP_000813794.1
• Coordinates: 1990770..1990946 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HZT27_RS09600
• Protein ID: WP_001270286.1
• Coordinates: 1990992..1991408 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT27_RS09575 (1986389)	1986389..1987564	-	1176	WP_001236265.1	BenE family transporter YdcO	-
HZT27_RS09580 (1987656)	1987656..1988192	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HZT27_RS09585 (1988265)	1988265..1990226	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HZT27_RS09590 (1990318)	1990318..1990548	-	231	WP_000494244.1	YncJ family protein	-
HZT27_RS09595 (1990770)	1990770..1990946	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HZT27_RS09600 (1990992)	1990992..1991408	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HZT27_RS09605 (1991487)	1991487..1992893	+	1407	WP_240767115.1	PLP-dependent aminotransferase family protein	-
HZT27_RS09610 (1993138)	1993138..1994283	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HZT27_RS09615 (1994301)	1994301..1995314	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HZT27_RS09620 (1995315)	1995315..1996256	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T167688 WP_000813794.1 NZ_CP058846:1990770-1990946 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T167688 NZ_CP075343:c2117123-2116842 [Streptomyces sp. EMB24]
ATGGGATGCGTCACGCGCTTCACGCCGCACGCGCAGCGGGACATGCTCAAGATCCCGCGCCCGGACGCCCTGCGGATCCT
GTATCGGTTCACCGAGTTGCAGAAGGCGATGGACGCCGGCGACACAGCGGCGTTCGACATCAAGGCCCTGCAGGGCCACA
GTGCCCGGTGGCGGCTCAGGGTCGGTGACTACCGCGTCGTCTACACCGTGGAGGACGGTCGGCTGATCGTGTGGGCCCTG
GCTCTCGGCAATCGCCTGGACGTCTACCGTCAGGTGCCCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT167688 WP_001270286.1 NZ_CP058846:1990992-1991408 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT167688 NZ_CP075343:c2117409-2117128 [Streptomyces sp. EMB24]
ATGAGCGAACCGGTGATCGAATCCATGGCCGAGGTCCGAGGCCACCTCGCCGACGTCATCGACCGTGCCCGGCGGGAGGA
GACCCCGACGATCATCACCCGGCGCGGGAAGCAGGAGGCCGTCGTGATCGACATCGAGGAGTACCAGCGGCTGCGCCGGA
TCGCCGAGAACGCCGAAGAGGCTTGGCTCAACCGGCTGGCCGACGAAGCCGAGTCCGAAGGCCTGGAGGGGGCGGTCTCG
CTCGAGGAGATGGCCGCCATGCTCCGCACCGACCGGGACTGA

Structures

Toxin

• PDB: 6HPB