167682

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 964532..965369 Replicon chromosome
Accession NZ_CP058846
Organism Shigella flexneri strain STLEFF_35

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag HZT27_RS04580 Protein ID WP_000227786.1
Coordinates 964532..965074 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HZT27_RS04585 Protein ID WP_001297137.1
Coordinates 965058..965369 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT27_RS04560 (960071) 960071..960982 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
HZT27_RS04565 (961150) 961150..961641 + 492 WP_001138904.1 nucleotide binding protein YajQ -
HZT27_RS04570 (961769) 961769..963133 - 1365 WP_001000978.1 MFS transporter -
HZT27_RS04575 (963541) 963541..964476 + 936 WP_001297127.1 tetratricopeptide repeat protein -
HZT27_RS04580 (964532) 964532..965074 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
HZT27_RS04585 (965058) 965058..965369 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HZT27_RS04590 (965554) 965554..966444 - 891 WP_000971336.1 heme o synthase -
HZT27_RS04595 (966456) 966456..966785 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HZT27_RS04600 (966785) 966785..967399 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HZT27_RS04605 (967389) 967389..969380 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HZT27_RS04610 (969402) 969402..970349 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T167682 WP_000227786.1 NZ_CP058846:c965074-964532 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T167682 NZ_CP075329:c30520-30194 [Xanthomonas citri pv. citri]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT167682 WP_001297137.1 NZ_CP058846:c965369-965058 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT167682 NZ_CP075329:c30687-30517 [Xanthomonas citri pv. citri]
ATGGCAGGGCTGCGTCCGGTGCAAATTTGGGTGCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTG
TCGTCTTGCTGCACAAGCGGACATGGCGGATACCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCT
GGACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References