Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3722531..3723107 Replicon chromosome
Accession NZ_CP058839
Organism Shigella flexneri strain STLEFF_34

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZT26_RS18095 Protein ID WP_001297643.1
Coordinates 3722531..3722818 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HZT26_RS18100 Protein ID WP_000063156.1
Coordinates 3722805..3723107 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT26_RS18075 (3718784) 3718784..3720064 - 1281 WP_001037380.1 DUF445 domain-containing protein -
HZT26_RS18080 (3720105) 3720105..3721337 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
HZT26_RS18085 (3721483) 3721483..3721578 - 96 Protein_3536 dienelactone hydrolase family protein -
HZT26_RS18090 (3721635) 3721635..3722332 + 698 Protein_3537 IS1 family transposase -
HZT26_RS18095 (3722531) 3722531..3722818 + 288 WP_001297643.1 BrnT family toxin Toxin
HZT26_RS18100 (3722805) 3722805..3723107 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HZT26_RS18105 (3723141) 3723141..3724097 - 957 WP_001297640.1 GTPase -
HZT26_RS18110 (3724108) 3724108..3724311 - 204 WP_000467859.1 YbdD/YjiX family protein -
HZT26_RS18115 (3724361) 3724361..3726511 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167670 WP_001297643.1 NZ_CP058839:3722531-3722818 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167670 NZ_CP075321:1065356-1065538 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT167670 WP_000063156.1 NZ_CP058839:3722805-3723107 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167670 NZ_CP075321:1065603-1066007 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References