Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2183165..2183819 Replicon chromosome
Accession NZ_CP058839
Organism Shigella flexneri strain STLEFF_34

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZT26_RS10665 Protein ID WP_000244777.1
Coordinates 2183165..2183572 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT26_RS10670 Protein ID WP_000354046.1
Coordinates 2183553..2183819 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT26_RS10645 (2179122) 2179122..2180855 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
HZT26_RS10650 (2180861) 2180861..2181571 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT26_RS10655 (2181596) 2181596..2182492 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZT26_RS10660 (2182604) 2182604..2183125 + 522 WP_001055874.1 flavodoxin FldB -
HZT26_RS10665 (2183165) 2183165..2183572 - 408 WP_000244777.1 protein YgfX Toxin
HZT26_RS10670 (2183553) 2183553..2183819 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT26_RS10675 (2184062) 2184062..2185042 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZT26_RS10680 (2185238) 2185238..2185897 - 660 WP_000250274.1 hemolysin III family protein -
HZT26_RS10685 (2186061) 2186061..2186372 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HZT26_RS10690 (2186417) 2186417..2187850 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HZT26_RS10695 (2187907) 2187907..2188650 - 744 WP_000951962.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167665 WP_000244777.1 NZ_CP058839:c2183572-2183165 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167665 NZ_CP075316:c5405905-5405522 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167665 WP_000354046.1 NZ_CP058839:c2183819-2183553 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167665 NZ_CP075316:c5406147-5405905 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References