Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1156943..1157774 Replicon chromosome
Accession NZ_CP058839
Organism Shigella flexneri strain STLEFF_34

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HZT26_RS05840 Protein ID WP_000854814.1
Coordinates 1157400..1157774 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag HZT26_RS05835 Protein ID WP_001285585.1
Coordinates 1156943..1157311 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT26_RS05800 (1152175) 1152175..1153245 + 1071 WP_000102643.1 patatin-like phospholipase family protein -
HZT26_RS05805 (1153381) 1153381..1154064 + 684 WP_000775500.1 hypothetical protein -
HZT26_RS05810 (1154080) 1154080..1154490 + 411 WP_000846713.1 hypothetical protein -
HZT26_RS05815 (1154711) 1154711..1155532 + 822 WP_001234530.1 DUF932 domain-containing protein -
HZT26_RS05820 (1155614) 1155614..1156093 + 480 WP_000860076.1 antirestriction protein -
HZT26_RS05825 (1156109) 1156109..1156585 + 477 WP_001186773.1 RadC family protein -
HZT26_RS05830 (1156648) 1156648..1156869 + 222 WP_000692323.1 DUF987 domain-containing protein -
HZT26_RS05835 (1156943) 1156943..1157311 + 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
HZT26_RS05840 (1157400) 1157400..1157774 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HZT26_RS05845 (1157771) 1157771..1157965 + 195 WP_000988600.1 DUF5983 family protein -
HZT26_RS05850 (1158011) 1158011..1158091 + 81 Protein_1151 hypothetical protein -
HZT26_RS05855 (1158380) 1158380..1158526 - 147 Protein_1152 transposase domain-containing protein -
HZT26_RS05860 (1158538) 1158538..1158762 + 225 WP_000917607.1 EutP/PduV family microcompartment system protein -
HZT26_RS05865 (1158863) 1158863..1159192 - 330 WP_000450409.1 DUF496 family protein -
HZT26_RS05870 (1159364) 1159364..1160422 - 1059 WP_001200905.1 FUSC family protein -
HZT26_RS05875 (1160620) 1160620..1161093 - 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
HZT26_RS05880 (1161212) 1161212..1162378 - 1167 WP_000830163.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T167661 WP_000854814.1 NZ_CP058839:1157400-1157774 [Shigella flexneri]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T167661 NZ_CP075316:4191423-4191641 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT167661 WP_001285585.1 NZ_CP058839:1156943-1157311 [Shigella flexneri]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT167661 NZ_CP075316:4191022-4191396 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References