Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34496..35021 Replicon plasmid unnamed1
Accession NZ_CP058834
Organism Shigella flexneri strain STLEFF_27

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HZT22_RS23485 Protein ID WP_001159871.1
Coordinates 34716..35021 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HZT22_RS23480 Protein ID WP_000813634.1
Coordinates 34496..34714 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT22_RS23460 (29658) 29658..30416 + 759 WP_000494510.1 hypothetical protein -
HZT22_RS23465 (30707) 30707..32230 + 1524 WP_000921251.1 hypothetical protein -
HZT22_RS23470 (32265) 32265..33389 + 1125 WP_000386705.1 DUF4238 domain-containing protein -
HZT22_RS23475 (33389) 33389..33679 + 291 WP_000648897.1 hypothetical protein -
HZT22_RS23480 (34496) 34496..34714 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HZT22_RS23485 (34716) 34716..35021 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HZT22_RS23490 (35022) 35022..35831 + 810 WP_000016963.1 site-specific integrase -
HZT22_RS23495 (36003) 36003..36356 + 354 WP_000864813.1 colicin M immunity protein -
HZT22_RS23500 (36406) 36406..36975 - 570 Protein_42 lipid II-degrading bacteriocin colicin M -
HZT22_RS23505 (37032) 37032..37729 + 698 WP_240763292.1 IS1 family transposase -
HZT22_RS23510 (37887) 37887..38759 - 873 WP_000794337.1 hypothetical protein -
HZT22_RS23515 (38990) 38990..39687 + 698 WP_223200906.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cmlA1 / qacE / blaCTX-M-14 / erm(B) / mph(A) - 1..98962 98962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T167647 WP_001159871.1 NZ_CP058834:34716-35021 [Shigella flexneri]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T167647 NZ_CP075286:c4842165-4841881 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT167647 WP_000813634.1 NZ_CP058834:34496-34714 [Shigella flexneri]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT167647 NZ_CP075286:c4842397-4842155 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References