Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4280715..4281333 Replicon chromosome
Accession NZ_CP058833
Organism Shigella flexneri strain STLEFF_27

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZT22_RS20810 Protein ID WP_001291435.1
Coordinates 4280715..4280933 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZT22_RS20815 Protein ID WP_000344800.1
Coordinates 4280959..4281333 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT22_RS20775 (4276004) 4276004..4276576 + 573 WP_000779831.1 YbaY family lipoprotein -
HZT22_RS20780 (4276607) 4276607..4276918 - 312 WP_000409911.1 MGMT family protein -
HZT22_RS20790 (4277297) 4277297..4277650 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HZT22_RS20795 (4277692) 4277692..4279242 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT22_RS20800 (4279406) 4279406..4279876 - 471 WP_000136192.1 YlaC family protein -
HZT22_RS20805 (4279992) 4279992..4280543 - 552 WP_000102564.1 maltose O-acetyltransferase -
HZT22_RS20810 (4280715) 4280715..4280933 - 219 WP_001291435.1 HHA domain-containing protein Toxin
HZT22_RS20815 (4280959) 4280959..4281333 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZT22_RS20820 (4281879) 4281879..4285028 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HZT22_RS20825 (4285051) 4285051..4286244 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167646 WP_001291435.1 NZ_CP058833:c4280933-4280715 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167646 NZ_CP075286:c4740839-4740306 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167646 WP_000344800.1 NZ_CP058833:c4281333-4280959 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167646 NZ_CP075286:c4741116-4740850 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References