Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3723731..3724307 Replicon chromosome
Accession NZ_CP058833
Organism Shigella flexneri strain STLEFF_27

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZT22_RS18080 Protein ID WP_001297643.1
Coordinates 3723731..3724018 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HZT22_RS18085 Protein ID WP_000063156.1
Coordinates 3724005..3724307 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT22_RS18060 (3719984) 3719984..3721264 - 1281 WP_001037380.1 DUF445 domain-containing protein -
HZT22_RS18065 (3721305) 3721305..3722537 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
HZT22_RS18070 (3722683) 3722683..3722778 - 96 Protein_3535 dienelactone hydrolase family protein -
HZT22_RS18075 (3722835) 3722835..3723532 + 698 Protein_3536 IS1 family transposase -
HZT22_RS18080 (3723731) 3723731..3724018 + 288 WP_001297643.1 BrnT family toxin Toxin
HZT22_RS18085 (3724005) 3724005..3724307 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HZT22_RS18090 (3724341) 3724341..3725297 - 957 WP_001297640.1 GTPase -
HZT22_RS18095 (3725308) 3725308..3725511 - 204 WP_000467859.1 YbdD/YjiX family protein -
HZT22_RS18100 (3725561) 3725561..3727711 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167643 WP_001297643.1 NZ_CP058833:3723731-3724018 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167643 NZ_CP075286:1960905-1961007 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT167643 WP_000063156.1 NZ_CP058833:3724005-3724307 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167643 NZ_CP075286:c1961013-1960869 [Klebsiella pneumoniae]
ACATATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References