Detailed information of TA system TA167637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2046981..2047564
• Replicon: chromosome
• Accession: NZ_CP058833
• Organism: Shigella flexneri strain STLEFF_27

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HZT22_RS10035
• Protein ID: WP_049253269.1
• Coordinates: 2046981..2047316 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: HZT22_RS10040
• Protein ID: WP_000581941.1
• Coordinates: 2047316..2047564 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT22_RS10020 (2042868)	2042868..2044166	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
HZT22_RS10025 (2044254)	2044254..2045891	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HZT22_RS10030 (2046119)	2046119..2046910	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
HZT22_RS10035 (2046981)	2046981..2047316	-	336	WP_049253269.1	endoribonuclease MazF	Toxin
HZT22_RS10040 (2047316)	2047316..2047564	-	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HZT22_RS10045 (2047642)	2047642..2049876	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
HZT22_RS10050 (2049924)	2049924..2051225	-	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T167637 WP_049253269.1 NZ_CP058833:c2047316-2046981 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T167637 NZ_CP075261:23624-23929 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT167637 WP_000581941.1 NZ_CP058833:c2047564-2047316 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT167637 NZ_CP075261:23404-23622 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCTGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9